La definición de juvenil, por lo tanto, se basa tácitamente más en la ausencia de algo en relación al adulto (madurez sexual, tamaño máximo, etc.) que en una caracterización morfológica particular. Este criterio ha sido seguido por numerosos autores que definen diferentes estadios juveniles sobre la base del grado de osificación, la microhistología ósea, cantidad de vascularización de los huesos y/o ausencia de fusión de epífisis y diáfisis (Banbury y Maglia, 2006 Horner et al., 2000 Maisano, 2002), patrones de coloración (Pérez i de Lanuza et al., 2012) o simplemente en un tamaño sustancialmente menor al que se ha señalado como máximo en una especie (Ponssa et al., 2010 Streicher et al., 2012 Lange et al., 2012 Esperón Rodriguez y Gallo Reynoso, 2013 Vera y Ponssa, 2014 Vera et al., 2015). De Beer (1940) distingue las estructuras juveniles, como todo aquello que aparece o se desarrolla previo al estadio adulto, considerando esto último como la adquisición de la forma y tamaño final. Evidence found, let us consider that specimens studied belong to one species of the Lithobates montezumae subgroup, distributed principally in the Southern Mexican Plateau. The PCA indicates that variables are not linearly correlated and specimens belong to a single group. Phylogenetic hypothesis shows an exclusive group with a previous sequence of Lithobates montezumae. Partial sequences of the 12S rDNA obtained from frogs distributed in the Southern Mexican Plateau show two haplotypes with a divergence of 0.4%. In addition, 20 morphological lineal measurements from 97 specimens were analyzed by principal component analysis (PCA) to evaluate if the frogs studied are conspecific. A phylogenetic analysis using partial sequences of 12S rDNA mitochondrial gene for 31 samples was carried out using Bayesian Inference, Maximum Parsimony, and Maximum Likelihood. In this study, we used 12S ribosomal RNA gene partial sequences to identify specimens of leopard frogs from some new localities (not sampled on previous studies) of the Southern Mexican Plateau, to carry out a phylogenetic analysis, and also a morphometric analysis of some morphological features were analyzed to evaluate their morphological variation. Species of the genus Lithobates are difficult to identify, especially on the ‘Rana pipiens’ complex, because the morphological differences among the species are often subtle. chiricahuensis populations in the United States. In light of this, a more comprehensive examination may yield valuable conservation insights and have implications for the management of declining L. Our field observations from 2008 suggest that this population is large and thriving. Additionally, we observed notable amounts of color pattern variation in female specimens. Multivariate analyses of 21 size-adjusted body measurements revealed putative shifts in body dimensions associated with gender and ontogeny. To better characterize this poorly known population, we examined morphological variation in 30 individuals that included adult males, adult females, and subadult females.
chiricahuensis from the Mexican state of Durango. We used mitochondrial and nuclear DNA to identify a population of L. Although extensive research has been conducted on populations in the United States, the status of most Mexican populations is unknown. In the United States, it is listed as a federally threatened species. The Chiricahua Leopard Frog (Lithobates chiricahuensis) occurs in parts of the southwestern United States and northwestern Mexico.
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